Techniques used to study the DNA polymerase reaction pathway.
نویسنده
چکیده
A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.
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عنوان ژورنال:
- Biochimica et biophysica acta
دوره 1804 5 شماره
صفحات -
تاریخ انتشار 2010